Abstract

Objective: The present study interpreted the prevalence of NFGNB from clinical specimens and assessed antibiotic resistance pattern as well as incidence of Extended Spectrun Beta lactamase(ESBL) and Metallo Beta Lactamase(MBL) producers. Methods: A total of 3585 clinical specimens were included and all the samples were processed for routine bacterial culture and antimicrobial susceptibility test as per standard protocol. They were further subjected to ESBL production detection by phenotypic confirm atory double disk diffusion test using cefotaxime with and without clavulanic acid. Imipenem resistant isolates were selected for the detection of MBL production by Imipenem-EDTA combined disc test. All the specimens were identified by the classical Microb iological and Biochemical tests. Results: The result showed that 304 non fermenting gram negative bacilli were isolated from various clinical specimens. Out of 304 NFGNB,195 (64.1%) isolates were Pseudomonas spp. followed by Acinetobacter spp. i.e 109 (35. 9%). Imipenem (85.1% sensitive) were the most effective drug against P. aeruginosa where as Acinetobacter spp. were most sensitive to colistin and Imipenem with 76.1% and 18.3% sensitivity respectively. Altogether, 69.1% of total NFGNB isolates were MDR an d ESBL and MDR produces were determined to be 51.97% and 21.4% respectively. Forty five (14.8%) of the total isolated were found to produce both ESBL and MBL. Conclusions: The study showed increasing trend of MDR, ESBL and MBL production in NFGNB so constant survey of antibiotic sensitivity and precautionary use of antibiotics is essential to control and manage spread of these isolates in different units of health institutions.